Laboratory Procedures and Practices for Intracytoplasmic Sperm Injection (ICSI)

Laboratory Procedures and Practices for Intracytoplasmic Sperm Injection (ICSI)


Goral Gandhi


4 DAYS            


₹120,000 + Taxes

Train one-on-one with our comprehensive hands-on training in the Intracytoplasmic Sperm Injection (ICSI) techniques. Learn  the Micromanipulation Process extensively and get an opportunity to work with ICSI workstation under the supervision of experts. Know the ICSI workstation, its set up, prepare the dishes and align the pipettes. Hours of hands-on practice in catching the sperms and injecting the oocytes.  The best practices of ICSI and ways to achieve the high fertilization rates and low degeneration rates will be taught as key expert tips will be imparted. This course provides a one-on-one training to develop knowledge and hands-on skills in all aspects of micromanipulation for ICSI.

Course Objectives

  What will you learn?

During the course each participant receives one-on-one training to :

  • Acquire proficiency in sperm preparation of ejaculated sperm for ICSI
  • Double density gradient technique
  • Swim-up technique
  • Acquire proficiency in preparation of dishes for ICSI
  • Acquire proficiency in handling oocytes for ICSI
  • Acquire proficiency in selection and catching of sperms for ICSI
  • Acquire proficiency in injecting the oocytes and the actual ICSI procedure
  • Trouble shooting tips for ICSI using KPIs


Laboratory Training

The syllabus covers the following areas :

 Equipment & Tools

  • Micromanipulators: Components and mechanics
  • Installing and aligning the ICSI instruments

 IVF and ICSI Techniques

  • Sperm preparation techniques for ICSI
  • Preparation of ICSI dishes
  • Oocyte handling for ICSI
  • Demonstration of denudation through videos
  • ICSI: Selection of sperms
  • ICSI: Immobilization and catching of sperms
  • ICSI : Injection of the spermatozoon into the oocyte

Educational Teaching Methods 

  • Interactive discussion and teaching sessions
  • Hands-on laboratory training sessions
  • Live lectures and multimedia presentations

Evaluation Of Trainee 

  • Knowledge/Theoretical Component (30%): Multiple choice questions on lab techniques linked to each course module
  • Practical Skills (50%): Tested on specific lab techniques taught during the training
  • Verbal Communication & Comprehension Skills / Integrative Component (20%): PowerPoint talk on a topic linked to a course module and viva voce
  • The performance of the candidate throughout the course will be reflected in a Report
  • Candidates who meet the training requirements and successfully complete the course work will be awarded the Training Certificate

Each participant will receive : 

  • Course Syllabus with Reading Material and Lab Protocols
  • Certificate of Training (issued upon successful assessment by the instructor)
  • Daily Lunch
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